IVF Treatment

Step 1: Ovarian Stimulation

Ovarian stimulation is the first step in an IVF cycle. It involves a series of daily follicle-stimulating hormone (FSH) injections self-administered at home from Day 2 or 3 of your menstrual cycle. This medication is used to stimulate the ovaries to encourage the growth of multiple follicles containing eggs.

Step 2: Control & Monitoring

Your fertility specialist will closely monitor your ovarian response during the stimulation phase with ultrasounds and/or blood tests. This is important as medications may be adjusted to control the size and amount of follicles.

Step 3: Egg Release

To assist with the final maturation of the egg and loosening of the egg from the follicle wall ready for egg collection, an injection of Human Chorionic Gonadotrophin (hCG). This is often referred to as ‘the trigger injection’.

Step 4: Egg Retrieval

The egg retrieval is performed 35-38 hours after ‘the trigger injection’ under ultrasound guidance and takes place while you’re lightly sedated. It is important to note that not every follicle will contain an egg, and not every egg will fertilise.

Step 5: Insemination

Your partner (if applicable) will be asked to produce his semen sample on the day of your procedure. If you are using frozen sperm, the sample is thawed on the day of your procedure. The sperm sample is washed and concentrated, then added to the eggs. If your specialist has recommended Intracytoplasmic Sperm Injection (ICSI), one sperm will be injected directly into each mature egg. ICSI is usually recommended for male factor infertility or previous suboptimal outcomes with standard IVF insemination.

Step 6: Fertilisation

The inseminated eggs are placed in an incubator and checked for fertilisation 16-18 hours after insemination. You will receive an update from the embryologists at this time. The normally fertilised embryos are then grown for five days. It is important to note that not every egg will fertilise, as several factors may affect the fertilisation ability of an egg and/or sperm.

Step 7: Embryo Transfer

Five days following egg retrieval, the embryos are assessed by the embryologists for development and quality. The best embryo is selected for transfer into the uterus using a fine plastic tube (transfer catheter). Usually only one embryo is transferred. If more than one embryo is to be transferred, this must be discussed with your clinician prior to transfer taking place. Embryo transfer is a relatively quick procedure and discomfort should be minimal.

In addition to fresh embryo transfers, many patients may have embryos frozen for future use. This process is known as a Frozen Embryo Transfer (FET), where a cryopreserved embryo from a previous cycle is thawed and placed into the uterus is widely used in IVF treatment. FETs avoid the need for another full IVF cycle, reducing treatment time, costs, and physical demands. Learn more about how FET works and if it may be right for you on our Frozen Embryo Transfer (FET) service page.

Step 8: Vitrification

Any remaining embryos that meet the requirements in terms of development and quality will be frozen. These frozen embryos are then stored until required for a subsequent transfer. The vast majority of frozen embryos survive the freezing process and pregnancy rates are comparable with transfer of fresh embryos.

Step 9: Pregnancy Test

The final stage of IVF is called the luteal phase and it’s the two weeks between the egg collection and the pregnancy test. Many people find this to be the most difficult time to endure. Whilst it’s an exciting and optimistic time of the treatment cycle, it can often be emotionally charged with expectation and anxiety. You will be encouraged to limit your activity for 24 hours after the embryo transfer, eat well, stay hydrated and keep busy while you wait for your pregnancy blood test.